Family‐based linkage and association mapping reveals novel genes affecting Plum pox virus infection in Arabidopsis thaliana
2012
Summary Sharka is a devastating
viral diseasecaused by the Plum pox virus (PPV) in stone
fruit treesand few sources of resistance are known in its natural hosts. Since any knowledge gained from Arabidopsis on
plant virussusceptibility factors is likely to be transferable to crop species, Arabidopsis's natural variation was searched for
host factorsessential for PPV infection. To locate regions of the genome associated with susceptibility to PPV, linkage analysis was performed on six biparental populations as well as on multiparental lines. To refine
quantitative trait locus(QTL) mapping, a genome-wide association analysis was carried out using 147 Arabidopsis accessions. Evidence was found for linkage on chromosomes 1, 3 and 5 with restriction of PPV long-distance movement. The most relevant signals occurred within a region at the bottom of
chromosome 3, which comprises seven RTM3-like TRAF domain-containing genes. Since the resistance mechanism analyzed here is recessive and the rtm3 knockout mutant is susceptible to PPV infection, it suggests that other gene(s) present in the small identified region encompassing RTM3 are necessary for PPV long-distance movement. In consequence, we report here the occurrence of
host factor(s) that are indispensable for virus long-distance movement.
Keywords:
-
Correction
-
Source
-
Cite
-
Save
54
References
32
Citations
NaN
KQI