Fungal Microbiota Profile in Newly Diagnosed Treatment-naïve Children with Crohn’s Disease
2016
Background & Aims: although increasing evidence suggests a role for fungi in inflammatory bowel disease (IBD), data are scarce and mostly from adults. Our aim was to define the characteristics of fungal microbiota in newly-diagnosed treatment-naive children with Crohn disease (CD). Methods: The children referred for colonoscopy were prospectively enrolled in the study at King Khalid University Hospital, King Saud University and Al Mofarreh
Polyclinicsin Riyadh. Tissue and
stool sampleswere collected and frozen till sequencing analysis. The children with confirmed CD diagnosis were designated as cases and the others as non- IBD controls. 78 samples were collected from 35 children (15 CD and 20 controls). Statistical analysis was performed to investigate CD associations and diversity. Results: CD-associated fungi varied with the level of phylogenetic tree. There was no significant difference in abundance between normal and inflamed mucosa. Significantly abundant CD-associated taxa included
Psathyrellaceae(p=0.01),
Cortinariaceae(p= 0.04),
Psathyrella(p= 0.004), and
Gymnopilus(p=0.03).
Moniliniawas significantly depleted (p=0.03), whereas other depleted taxa, although not statistically significant, included
Leotiomycetes(p= 0.06),
Helotiales(p=0.08),
Sclerotiniaceae(p=0.07). There was no significant difference in
fungal diversitybetween CD and controls. Conclusions: We report highly significant fungal
dysbiosisin newly diagnosed treatment naive CD children. Depleted and more abundant taxa suggest anti-inflammatory and proinflamatory potentials respectively. Further studies with larger sample size including functional analysis are needed to clarify the significance of the fungal community in the pathogenesis of CD.
Keywords:
-
Correction
-
Source
-
Cite
-
Save
39
References
23
Citations
NaN
KQI