Purification, compositional analysis, and anticoagulant capacity of chondroitin sulfate/dermatan sulfate from bone of corb (Sciaena umbra)

Abstract Chondroitin sulfate/ dermatan sulfate(CS/DS) were isolated and purified for the first time from the bone of corb ( Sciaena umbra) (CBG) and their chemical composition and anticoagulant activity were assessed. Infrared spectrum and agarose-gel electrophoresisfor extracted CS/DS were also investigated. The results showed that the purified CS/DS obtained at a yield of 10% contains about 31.28% sulfate and an average molecular mass of 23.35 kDa. Disaccharideanalysis indicated that CBG was composed of monosulfated disaccharidesin positions 6 and 4 of the N- acetylgalactosamine(8.6% and 40.0%, respectively) and disulfated disaccharidesin different percentages. The charge density was 1.4 and the ratio of 4:6 sulfated residues was equal to 4.64. Chondroitinase AC showed that the purified CS/DS contained mainly 74% CS and 26% DS. Moreover, the new CS/DS extracted from bone of corb showed a strong anticoagulant effect through activated partial thrombosis time (aPTT), thrombin time(TT) and prothrombin time(PT). In fact, CBG prolonged significantly ( p