Whole genome expression profiling of normal human fetal and adult ocular tissues
2013
To study growth and development of ocular tissues, gene expression patterns in normal human fetal versus adult eyes were compared. Human retina/
retinal pigment epithelium, choroid,
sclera,
optic nerve* and cornea* tissues were dissected from fetal (24 week gestational age) (N = 9; *N = 6), and adult (N = 6) normal donor eyes. The Illumina® whole genome expression microarray platform was used to assess differential expression. Statistical significance for all comparisons was determined using the Benjamin and Hochberg
False Discovery Rate(FDR, 5%). Significant gene expression
fold changes> 1.5 were found in adult versus fetal retina/RPE (N = 1185), choroid (N = 6446),
sclera(N = 1349), and cornea (N = 3872), but not
optic nerve. Genes showing differential expression were assessed using
Ingenuity
Pathway Analysis(IPA) for enriched functions and canonical pathways. In all tissues, development, cell death/growth, cancer functions, and signaling canonical pathways were enriched. There was also a general trend of down-regulation of collagen genes in adult tissues.
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