RNA Profiling of the Human and Mouse Spinal Cord Stem Cell Niches Reveals an Embryonic-like Regionalization with MSX1+ Roof-Plate-Derived Cells
2019
Summary
Anamniotes, rodents, and young humans maintain
neural stem cellsin the ependymal zone (EZ) around the central canal of the spinal cord, representing a possible endogenous source for repair in mammalian lesions. Cell diversity and genes specific for this region are ill defined. A cellular and molecular resource is provided here for the mouse and human EZ based on RNA profiling, immunostaining, and fluorescent transgenic mice. This uncovered the conserved expression of 1,200 genes including 120
transcription factors. Unexpectedly the EZ maintains an embryonic-like dorsal-ventral pattern of expression of spinal cord developmental
transcription factors(ARX,
FOXA2, MSX1, and
PAX6). In mice, dorsal and ventral EZ cells express Vegfr3 and are derived from the embryonic roof and
floor plates. The dorsal EZ expresses a high level of Bmp6 and
Gdf10genes and harbors a subpopulation of radial quiescent cells expressing MSX1 and ID4
transcription factors.
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