Molecular characterization of two isoforms of defensin from hemocytes of the oyster Crassostrea gigas
2007
Antimicrobial peptides(AMPs) are important components of the host innate immune response against microbial invasion. We previously characterized the first AMP from an
oyster, a
defensin, that was shown to be continuously expressed in the mantle of
Crassostrea
gigas. In this study, we report the cDNA cloning of two new isoforms of the
defensinAMP family (Cg-defh1 and Cg-defh2) from the
hemocytesof the
oyster. The deduced amino acid sequences reveal two peptides of 73 amino acid residues with a mature portion consisting of 43 amino acid residues. Cg-Defh1 and Cg-Defh2 share 86% amino acid identity and belong to the “arthropod-molluscs
defensinfamily”. qRT-PCR analyses indicate that Cg-defh2 is continuously expressed in the
hemocytesof C.
gigas. In addition, after a bacterial challenge, the level of Cg-defh2 transcripts decreases dramatically in the circulating
hemocytepopulation and this decrease can be correlated with an increase of Cg-defh2 transcripts in the gill and the mantle tissue, suggesting a possible migration of the
hemocytesexpressing Cg-defh2 towards the tissues implicated in the first defense barrier of the
oyster. These results would suggest an important role of Cg-Defh2 in the
oysterresponse to a microbial challenge.
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