Hydrophilic Polystyrene-Polyoxyethylene Graft Polymer Beads as Carrier of Antigenic Peptides for in vivo and in vitro Immunization Techniques: Applications to the Non-Catalytic Zinc Loop of HLADH Isozymes and to Histone Fragments

Polystyrene-polyethyleneglycol tentacle polymers (PS-PEG) are a novel class of graft copolymer beads based on a polystyrene matrix grafted with a brush-like layer of polyoxyethylene tentacles. Immunogenic materials were obtained by synthesizing on tentacle polymer-bound amino derivatives as a solid support the following haptens: The C-terminal sequence 187-211 KPKAA KPKAA KPKAA KPKAA KPKKA APKK and the N-terminal sequence 3-11 APAAP AAAPP AEKTP VKKKA AKKKPA GA of histone H1 (calf thymus), as well as the sequences 93-116 of the horse liver alcohol dehydrogenase EE (FTPQC GKCRV CKHPE GNFCL KNDL) and SS (FIPQC GKCSV CKHPE GNLCL KNSL) isozymes