Inhibitory Activity of Fruits Extracts of A n t i d e s m a b unius on the Proliferation and Migration of MDA-MB-231 Breast Cancer Cells

In this research, we investigated the inhibitory effect of the ethanolic (EtOH) extract of bignay (Antidesma bunius) fruit on the viability of breast cancer cells (MDA-MB-231) by CCK-8 assay. The results showed that the half inhibitory concentration (IC50) was 219 μg/mL after the cells were treated with EtOH extract for 72 hours. The EtOH extract was sequentially extracted by petroleum ether (PET), ethyl acetate (EtOAc), and n-butanol (nBuOH). Among them, the EtOAc fraction showed higher anti-proliferative activity than the other fractions. Wound healing experiment showed the EtOAc fraction inhibited the migration of MDA-MB-231 cells and significantly delayed the transition of the G1 to S phase compared to control. UHPLC-MS/MS analysis showed that in addition to citric acid and phenolic acids (3-Hydroxybenzoic acid, aspirin, fertaric acid), EtOH extract of bignay fruit contains active ingredient like coumarin, genistin, amentoflavone, and luteolin 7-galactoside. The anti-proliferation activity of amentoflavone against MDA-MB-231 cells was also confirmed.