Melatonin from cerebrospinal fluid but not from blood reaches sheep cerebral tissues under physiological conditions.
2014
The
pineal glandsecretes
melatoninthat circulates in the blood and
cerebrospinal fluid. We provide data that support the hypothesis that, in sheep and maybe in human, only the
cerebrospinal fluid
melatonin, and not the blood
melatonin, can provide most of
melatoninto the cerebral tissue in high concentrations, particularly in the periventricular area. The
melatonincontent of sheep brain, our chosen animal model, was found in significant concentration gradients oriented from the
ventricle(close to the
cerebrospinal fluid) to the cerebral tissue, with concentrations varying by a factor of 1 to 125. The highest concentrations were observed close to the
ventriclewall, whereas the lowest concentrations were furthest from the
ventricles(407.0 ± 71.5 pg/ml compared to 84.7 ± 5.2 pg/ml around the
third ventricle). This concentration gradient was measured in brain tissue collected at mid-day and at the end of the night. Nocturnal concentrations were higher than daytime concentrations, reflecting the diurnal variation in the
pineal gland. The concentration gradient was not detected when
melatoninwas delivered to the brain via the bloodstream. The diffusion of
melatoninto cerebral tissues via
cerebrospinal fluidwas supported by in vivo scintigraphy and autoradiography. 2-[(123) I]-
melatonininfused into the
cerebrospinal fluidquickly and efficiently diffused into the brain tissues, whereas [(123) I]-iodine (control) was mostly washed away by the
cerebrospinal fluidflow and [(123) I]-BSA remained mostly in the
cerebrospinal fluid. Taken together, these data support a critical role of
cerebrospinal fluidin providing the brain with
melatonin.
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