A POSSIBLE ROLE OF THE PANCREATIC α1- AND α2-CELLS AS LOCAL REGULATORS OF INSULIN SECRETION*

SUMMARY In addition to a majority of insulin-producing β-cells, the mammalian pancreatic islets contain considerable numbers of α 1 - and α 2 -cells. While the α 2 -cells are the cellular source of glucagon, the secretory product stored in the granules of the α 1 -cells still remains to be identified. Several authors have reported that glucagon stimulates insulin release. This effect of glucagon was found to be dependent on the presence of adequate amounts of glycogen in the β-cells. The resulting insensitivity of the β-cells to glucagon under conditions of hypoglycemia is consistent with the previous concept of glucagon as a local regulator of the β-cell function. The α 1 -cells may also be considered as possible regulators of insulin secretion from the adjacent β-cells. The α 1 -cells are morphologically even more closely related to the β-cells than are the α 2 -cells. After measuring the amounts of insulin secreted from microdissected mouse islets under different experimental conditions it could be concluded that there exists at least one inhibitor of insulin release within the pancreatic islets. Such an inhibitor is probably located to the α 1 -cells, as shown by the fact that a water extract of pigeon α 1 -cells significantly reduced the amounts of insulin secreted in vitro. The supposed inhibitor of insulin release in the pancreatic α 1 -cells may be identical with gastrin, which inhibits the glucose-stimulated insulin release when tested at a concentration of only 0.15 μg/ml.