PH20 is not expressed in murine CNS and oligodendrocyte precursor cells

Mathieu Marella,Joe Ouyang,Jonathan Zombeck,Chunmei Zhao,Lei Huang,Robert James Connor,Kim Phan,Michael C. Jorge,Marie A. Printz,Rudolph D. Paladini,Arnold B. Gelb,Zhongdong Huang,Gregory I. Frost,Barry J. Sugarman,Lawrence Steinman,Ge Wei,H. Michael Shepard,Daniel C. Maneval,Paula J. Lapinskas

PH20 is not expressed in murine CNS and oligodendrocyte precursor cells

2017

Mathieu Marella
Joe Ouyang
Jonathan Zombeck
Chunmei Zhao
Lei Huang
Robert James Connor
Kim Phan
Michael C. Jorge
Marie A. Printz
Rudolph D. Paladini
Arnold B. Gelb
Zhongdong Huang
Gregory I. Frost
Barry J. Sugarman
Lawrence Steinman
Ge Wei
H. Michael Shepard
Daniel C. Maneval
Paula J. Lapinskas

Objective Expression of Spam1/PH20 and its modulation of high/low molecular weight hyaluronan substrate have been proposed to play an important role in murine oligodendrocyte precursor cell (OPC) maturation in vitro and in normal and demyelinated central nervous system (CNS). We reexamined this using highly purified PH20.

Keywords:

Oligodendrocyte progenitor
Molecular biology
Biology
In vitro
In vivo
Chemistry
Recombinant DNA
Immunolabeling
Antibody
Cell biology
Basic fibroblast growth factor
Real-time polymerase chain reaction
Messenger RNA

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