Conditional mutagenesis by oligonucleotide-mediated integration of loxP sites in zebrafish.
2018
Many eukaryotic genes play essential roles in multiple biological processes in several different tissues. Conditional mutants are needed to analyze genes with such
pleiotropicfunctions. In vertebrates, conditional gene inactivation has only been feasible in the mouse, leaving other model systems to rely on surrogate experimental approaches such as overexpression of dominant negative proteins and antisense-based tools. Here, we have developed a simple and straightforward method to integrate loxP sequences at specific sites in the zebrafish genome using the
CRISPR/
Cas9technology and oligonucleotide templates for
homology directed repair. We engineered conditional (
floxed) mutants of
tbx20and fleer, and demonstrate excision of exons flanked by loxP sites using tamoxifen-inducible CreERT2
recombinase. To demonstrate broad applicability of our method, we also integrated loxP sites into two additional genes,
aldh1a2and tcf21. The ease of this approach will further expand the use of zebrafish to study various aspects of vertebrate biology, especially post-embryonic processes such as regeneration.
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Correction
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