309 PROTEOMIC ANALYSIS OF THE BLASTOCOEL FLUID AND REMAINING CELLS OF BOVINE BLASTOCYSTS
2013
Human embryonic stem cells (hESC) are derived from the human
blastocystand possess the potential to differentiate into any cell type present in the adult human body. Human ESC are considered to have great potential in
regenerative medicinefor the future treatment of severe diseases and conditions such as Parkinson’s disease, diabetes, and spinal cord injury. One of today’s challenges in
regenerative medicineis to define proper culture conditions for hESC. The natural milieu in the
blastocystmay provide clues on how to improve culture conditions, and the aim of the present study was to determine the proteome of the
blastocoelfluid and the remaining cells of bovine
blastocysts. Bovine
blastocystswere produced by in vitro fertilization of oocytes retrieved from slaughterhouse ovaries. The
blastocoelfrom 195
blastocysts(1–8 nL per
blastocyst) were isolated by
micromanipulationand analysed by nano-HPLC tandem mass spectrometry along with the remaining cells of the
blastocyst. Searching the mass spectrometry data against a combined bovine database (SwissProt/TrEMBL), we identified 263 proteins in the
blastocoelfluid and 1606 proteins in the
cellular compartmentof the
blastocyst. A
Venn diagramshowed 124 proteins in overlap between the two compartments of the
blastocyst. Several heat shock proteins and specific antioxidants were identified in both the
blastocoeland cell material. A selection of proteins identified in the
blastocoelfluid is to be tested on hESC in cell culture experiments, with proliferation of undifferentiated cells as the primary endpoint. The results from this study provide new knowledge about early mammalian preimplantation development, and the data can be used in the continued pursue of improving culture conditions for hESC, which further facilitates the clinical application of these cells.
Keywords:
-
Correction
-
Source
-
Cite
-
Save
0
References
0
Citations
NaN
KQI