Nanomechanical aspects of brain metastasis formation and amyotrophic lateral sclerosis
2018
Atomic force microscopy-based
nanomechanicalmethods were directly applied in order to elucidate important questions related to
brain metastasisformation and amyotrophic lateral sclerosis (ALS). Force mapping with single cell
force spectroscopywas combined in order to gain direct insight into the surface “screening” process of tumor cells during their
extravasationinto the brain parenchyma. Furthermore, comparative SCFS measurements were performed between three melanoma cell types (WM35, A2058 and A375), showing altered invasive characteristics, and blood vessel lining endothelials in order to examine how the metastatic potential relates to tumor cell’s autonomous and inter-cellular
nanomechanicalproperties. In case of ALS by comparing the adhesion of CD8+
cytotoxic T cellsisolated from wild-type as well as SOD1G93A mutant mice against wild-type motor neurons we found that
cytotoxic T cellsare active contributors of neurodegeneration in the development of ALS. In addition,
nanomechanicalmapping of wild-type and SOD1G93A mutant mice derived single elongated myoblasts and multinuclear myotubes revealed differences in elasticity during early differentiation stage into myotubes. These findings provide valuable knowledge to the understanding of
brain metastasisformation and ALS pathogenesis.
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