Stressful times for women - Increased physiological stress in Neolithic females detected in tooth cementum

Abstract We used the tooth cementum annulation method (TCA) to investigate physiological stress before and during the Neolithic demographic transition in Europe. Episodes of physiological stress are reflected as “stress layers” in the tooth cementum at an almost annual resolution. We used the TCA method to detect and count the number of the stress events for a sample of 21 Mesolithic and 25 Neolithic individuals from the Central Balkans from the period between 9500 and 5400 years BC. In accord with the theory of the Neolithic demographic transition, we hypothesize that the Neolithic individuals will have more stress than the Mesolithic individuals. Our results suggest that the Neolithic females had significantly more stress layers in the tooth cementum per year of life than the Mesolithic females. The difference between Mesolithic and Neolithic males was not statistically significant. We conclude that Neolithic women had more physiological stress episodes than Mesolithic women. The differential pattern between sexes, combined with the fact that pregnancies are one of the major causes of stress layer formation in tooth cementum, might indicate that the observed differences are mostly due to increased fertility in the Neolithic.