HLAIb worldwide genetic diversity: New HLA-H alleles and haplotype structure description
2019
Abstract The classical
HLAclass I genes (
HLAIa) were extensively studied because of their implication in clinical fields and anthropology. Less is known about worldwide genetic diversity and linkage disequilibrium for non-classical
HLAclass I genes (
HLAIb) and
HLA
pseudogenes. Notably,
HLA-H, which is deleted in a fraction of the population, remains scarcely explored. The aims of this study were 1/ to get further insight into
HLA-H genetic diversity and into how this variability potentially affects its expression and 2/ to define
HLAIb worldwide allelic diversity and linkage.
Exomesequence data from the
1000 Genomes Projectwere used to define second field
HLA-A, -E, -F, -G and -H typing using PolyPheMe software. Allelic and two-loci haplotype frequencies were estimated using Gene[Rate] software both at worldwide and continental levels. Eleven novel
HLA-H alleles identified in
exomedata were validated by NGS performed on 25 genomic DNA samples from the same cohort. Phylogenetic analysis and frequency distribution of
HLA-H alleles revealed three clades, each predominantly represented in Admixed American, European and East Asian populations, African populations and South Asian populations. Among these eleven novel alleles, two potentially encode complete transmembrane
HLAproteins. We confirm the high LD between
HLA-H and -A, and between
HLA-H and -G , and show the three genes have distinct worldwide allelic distribution. Conversely,
HLA-E and
HLA-F both showed little LD, displayed restricted allelic diversity and practically no difference in their distribution across the planet. Our work thus reveals an unexpectedly high
HLA-H genetic diversity, with alleles highly represented in Asia possibly encoding a functional
HLAprotein. Functional implication of these results remains to be explored, both in physiological and pathological contexts.
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