Rapid screening of transferrin-binders in the flowers of Bauhinia blakeana Dunn by on-line high-performance liquid chromatography-diode-array detector-electrospray ionization-ion-trap-time-of-flight-mass spectrometry-transferrin-fluorescence detection system.
2016
Abstract
Transferrin(
Transferrin, TRF, TF) has drawn increasing attention in cancer therapy due to its potential applications in drug delivery. TF receptor, highly expressed in tumor cells, recognizes and transports Fe 3+ -TF into cells to release iron into cytoplasm. Thus, discovering TF-binding compounds has become an active research area and is of great importance for target therapy. In this study, an on-line analysis method was established for screening TF-binding compounds from the flowers of
Bauhinia blakeanaDunn using a high-performance liquid chromatography–diode-array detector–multi-stage mass spectrometry–
transferrin–fluorescence detector (HPLC–DAD–MS n –TF–FLD) method. As a result, 33 of 80 identified or tentatively characterized compounds in the sample were TF-binding active. Twenty-five
flavonolglycosides and eight
phenolic acidswere identified as TF-binders. Twelve of these active compounds together with six standard compounds were used to study the dose-response effects and structure-activity relationships of flavonoids and
phenolic acids. The method was validated by
vitexinwith a good linearity in the range of concentrations used in the study. The limit of detection for
vitexinwas 0.1596 nmol. Our study indicated that the established method is simple, rapid and sensitive for screening TF-binding active compounds in the extract of
Bauhinia blakeanaDunn, and therefore is important for discovering potential anti-cancer ingredients from complex samples for TF related drug delivery.
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