PULSin™: a potent reagent for protein delivery to eukaryotic cells

PULSin™ is a potent reagent for protein delivery to the cytoplasm of live eukaryotic cells. PULSin™ forms non-covalent complexes with proteins or antibodies. These complexes are internalized via anionic cell-adhesion receptors present on virtually all cells and are released into the cytoplasm where they disassemble. The native protein can thus diffuse throughout the cytoplasm and exert its function or proceed to its target organelle. PULSin™ delivers proteins to a large variety of live eukaryotic cells, including primary and suspension cells, without noticeable side effects. PULSin™-mediated antibody delivery is a convenient method for immunostaining of live cells and for protein interference studies. In this issue