Biodegradation of fungicide Tebuconazole by Serratia marcescens strain B1 and its application in bioremediation of contaminated soil
2018
Abstract The degradation characteristics of
Tebuconazoledegrading bacterium
Serratia marcescensstrain B1 in both contaminated soil and culture were investigated in this study. In the liquid mineral salt medium, the optimal temperature, pH value, and inoculation (v/v) for degradation by strain B1 were 30 °C, 7.0, and 4.0%, respectively. When the initial concentration of
Tebuconazolewas 200 mg L −1 , the degradation rate of
Tebuconazolewas 94.05% in 8 h. As the initial concentrations of
Tebuconazolewere higher than 300 mg L −1 , the biodegradation rates declined as the
Tebuconazoleconcentrations raised. As the concentration was 500 mg L −1 , the
Tebuconazolewas degraded at a rate of only 64.11%. Degradation fit well with the kinetic equation of
pesticide degradationas the initial concentrations of
Tebuconazolewere between 50 and 500 mg L −1 . When strain B1 (cultured at 3 × 10 14 CFU mL −1 ) was mixed with the
soil contaminatedwith tebuconzole (200 mg L −1 and the concentration of strain B1 of 3 × 10 7 CFU g −1 dry soil), the
Tebuconazolewas degraded at a rate of 96.46% in 30 days, while the control soil (with the absence of strain B1) achieved a degradation rate of only 70.42%. These results demonstrate that in contaminated soil, the strain B1 can substantially increase the degradation rate of
Tebuconazole. Results of the greenhouse and field experiments indicate that the strain B1 can remove the residues of
Tebuconazolein contaminated soil and Chinese cabbage.
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