A scalable double-barcode sequencing platform for characterization of dynamic protein-protein interactions
2017
Protein-protein interactions(PPIs) play a major role in defining
biological functions. Here, the authors present PPiSeq, a method to quantitatively score dynamic PPIs in yeast combining fragment complementation assays, genomic double-
barcoding, and an analytical framework to precisely call fitness from
barcodelineage trajectories.
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