Characterization of Human Cancer Cell Lines by Reverse-phase Protein Arrays
2017
Summary Cancer cell lines are major model systems for mechanistic investigation and drug development. However,
protein expression
data linkedto high-quality DNA, RNA, and drug-screening data have not been available across a large number of cancer cell lines. Using reverse-phase protein arrays, we measured expression levels of ∼230 key cancer-related proteins in >650 independent cell lines, many of which have publically available genomic, transcriptomic, and drug-screening data. Our dataset recapitulates the effects of mutated pathways on
protein expressionobserved in patient samples, and demonstrates that proteins and particularly
phosphoproteinsprovide information for predicting drug sensitivity that is not available from the corresponding mRNAs. We also developed a user-friendly bioinformatic resource, MCLP, to help serve the biomedical research community.
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