Application of Large-Scale Aptamer-Based Proteomic Profiling to Planned Myocardial Infarctions
2017
Background:Emerging
proteomictechnologies using novel affinity-based reagents allow for efficient multiplexing with high-sample throughput. To identify early biomarkers of myocardial injury, we recently applied an
aptamer-based
proteomicprofiling platform that measures 1129 proteins to samples from patients undergoing septal alcohol ablation for
hypertrophic cardiomyopathy, a
human modelof planned myocardial injury. Here, we examined the scalability of this approach using a markedly expanded platform to study a far broader range of human proteins in the context of myocardial injury. Methods:We applied a highly multiplexed, expanded
proteomictechnique that uses single-stranded DNA
aptamersto assay 4783 human proteins (4137 distinct human
gene targets) to derivation and validation cohorts of planned myocardial injury, individuals with spontaneous myocardial infarction, and at-risk controls. Results:We found 376
target proteinsthat significantly changed in the blood after planned myocardial injury in a...
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