An Engineered orco Mutation Produces Aberrant Social Behavior and Defective Neural Development in Ants

Summary Ants exhibit cooperative behaviors and advanced forms of sociality that depend on pheromone- mediated communication. Odorant receptor neurons (ORNs) express specific odorant receptors (ORs) encoded by a dramatically expanded gene family in ants. In most eusocialinsects, only the queen can transmit genetic information, restricting genetic studies. In contrast, workers in Harpegnathos saltatorants can be converted into gamergates(pseudoqueens) that can found entire colonies. This feature facilitated CRISPR- Cas9generation of germline mutationsin orco , the gene that encodes the obligate co-receptorof all ORs. orco mutations should significantly impact olfaction. We demonstrate striking functions of Orco in odorant perception, reproductive physiology, and social behavior plasticity. Surprisingly, unlike in other insects, loss of OR functionality also dramatically impairs development of the antennal lobeto which ORNs project. Therefore, the development of genetics in Harpegnathosestablishes this ant species as a model organismto study the complexity of eusociality.