Cryoprotective effect of glycerol against sperm DNA damage in frozen-thawed boar spermatozoa.

The use of frozen-thawed boar sperm still lags behind chilled boar semen in artificial insemination (AI) operations despite its advantages [1]. Freezing however, may damage the structure and function of sperm, leading to reduced fertilization success. Moreover, glycerol, which is a critical cryoprotectant in most sperm freezing protocols, can be toxic to cells in high concentrations [2]; affecting sperm motility and acrosomal integrity. This study was conducted to determine the cryoprotective effect of glycerol on sperm DNA integrity and motility in frozen-thawed boar sperm.