Expression of claudin-1 in canine peripheral nerve sheath tumours and perivascular wall tumours. Immunohistochemical study
2012
Aims: A peripheral nerve sheath tumour consists of neoplastic
Schwann cellsor perineurial cells, or a mixture of
Schwann cells, perineurial cells and fibroblasts. The first aim of the present study was to characterise the expression of the
claudin-1 tight junction protein in canine intact peripheral nerves, canine benign peripheral nerve sheath tumours (cBPNSTs), such as
schwannomas,
neurofibromas, perineuriomas and canine
malignant peripheral nerve sheath tumours(cMPNSTs), and in different other benign and malignant canine spindle cell tumours. The second aim of the present study was to examine whether
claudin-1 can help to distinguish the subgroups of canine perivascular wall tumours. Methods and results: The biopsy and necropsy samples (n=203) included 10 intact peripheral nerves, 20 cBPNSTs (4
schwannomas, 8
neurofibromas, 8 perineuriomas), 16 cMPNSTs, 6 psammomatous meningiomas, 6
dermatofibromas, 6 leiomyomas, 6
myxomas, 4
spindle cell hemangiomas, 2
spindle cell lipomas, 6 fibrohistiocytic nodules, 8
fibrosarcomas, 8
leiomyosarcomas, 6
myxosarcomas, 8
hemangiosarcomas, 8 anaplastic sarcomas, 8 amelanotic spindle cell melanomas, 8
histiocytic sarcomas, 8
spindle cell carcinomas, 8
myoepitheliomas, 8 complex carcinomas, 5 cardiac
rhabdomyosarcomas, 4
synovial sarcomas, 5 osteosarcomas, 4
chondrosarcomasand 4
liposarcomas; 31 canine perivascular wall tumours: 10
hemangiopericytomas, 8
myopericytomas, 6
angioleiomyomas, 4 angioleiomyosarcomas, 3
angiofibromas. The immunohistochemical panel consisted of
humanized antibodies: anti-
claudin-1, anti-neuron specific enolase, anti-S-100 protein, anti-?-smooth muscle actin, anti-vimentin, anti-cytokeratin AE1-AE3, anti-
claudin-5, anti-
Melan-A and anti-heavy
caldesmon, anti-
calponinand anti-
desmin. The intact perineurial cells, all perineuriomas,
neurofibromas, cMPNSTs,
spindle cell carcinomasand epithelial components of the complex carcinomas, all
hemangiopericytomasand myo-pericytomas showed
claudin-1 positivity. The
schwannomasand other spindle shape cell tumours were negative for
claudin-1. Conclusion: Our findings suggest that an antibody against
claudin-1, in combination with other antibodies, can be used as a novel diagnostic tool to differentiate canine peripheral nerve sheath tumours from other fusocellular tumours, and anti-
claudin-1, together with other antibodies, can also be used to subclassify cBPNSTs. Furthermore, analysis of
claudin-1 expression can help to differentiate between subgroups of canine perivascular wall tumours
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