Abstract A019: Establishment and characterization of a melanoma patient-derived xenograft model comprising three different sublines with distinct biologic features

Patient-derived tumor xenografts(PDX) play a major role in the development of new cancer therapies and their strengths and weaknesses have gradually been elucidated. Large panels of solid cancer PDX are available to screen innovative compounds, identify new targets, and study tumor biology. In the current study we established a melanoma PDX from donor patient tissue. In addition, we were able to create two sublines from spontaneous metastases occurring in the murine host during the establishment phase of the original model. All three lines were characterized by tumor growth kinetics, antitumoral activity against standard-of-care temozolomide, and pathohistologic examination. Furthermore, molecular examination of primary PDX and its metastases is under way as well as cell line establishment. The PDX model was developed from a biopsy of a 68-year-old woman undergoing surgery due to a non-pretreated melanoma. After seven subcutaneous passages in immune-compromised mice, individual animals showed tumor growth in the liver as well as the spleen. We were able to passage and characterize those metastases in parallel to the original model. The human origin of the lines as well as one cell line established in 2D from the primary PDX was confirmed by str-analysis. All three in vivo lines depicted distinct growth kinetics: The doubling times varied significantly (Kruskal-Wallis, p Citation Format: Kerstin Klingner, Dorothee Lenhard, Elke Simon, Anne-Lise Peille, Julia Schuler. Establishment and characterization of a melanoma patient-derived xenograft model comprising three different sublines with distinct biologic features [abstract]. In: Proceedings of the AACR-NCI-EORTC International Conference: Molecular Targets and Cancer Therapeutics; 2017 Oct 26-30; Philadelphia, PA. Philadelphia (PA): AACR; Mol Cancer Ther 2018;17(1 Suppl):Abstract nr A019.