Application of a Cancer Hotspot Panel to Guide Lung Cancer Therapy: Asian Experience

Background: Comprehensive genomic profiling (CGP) has transformed personalized medicine but is still not affordable to all patients, especially in developing countries. The price of CGP differs based on panel size. Aim: The aim of this study was to explore the benefits between a cancer hotspot panel and CGP in Asian lung cancer patients. Methods: One hundred sixty-two formalin-fixed, paraffin-embedded (FFPE) lung cancer clinical samples were subjected to next-generation sequencing using the Ion Torrent Proton System. Sixty-six cases were profiled using a 35-gene cancer hotspot panel and 96 with pathway-based CGP (400+ genes) for base substitutions (single nucleotide variants and small indels) and copy number variants (CNV). Results: In total, actionable variants were discovered in 86.4% (57/66) cases using the cancer hotspot panel, with 57.9% (33/57) derived from base substitutions, 10.5% (6/57) from CNVs and 31.6% (18/57) from both alterations. Contrarily, CGP identified actionability in 97.9% (94/96) pa...