배지조성별 동충하초 (Cordyceps militaris)의 Cordycepin 함량 비교

Background : Cordyceps militarishas been an wonder drug to anti-aging efficacy and called the three main drugs with ginseng and deer antlerfrom the past. Cordycepin, cordycepicacid ( d-mannitol) and adenosine are known as functional ingredientsin Cordyceps militaris. Among them, cordycepin, the representative component, has been reported as antimicrobial substance containing immune enhancement, anti-cancer and anti-inflammatory effects. Methods and Results : After Cordyceps militarisproduced from different types of medium mixed with 10-fold volume of purified water, the mixture were extracted at 70±5℃ for a hour and that extracts re-extracted using ultrasonics wave for 30 minutes. Qualitative analysis of the index component was determined by using the Q-TOF (A quadrupole time-of-flight mass spectrometer), and quantitative analysis was performed by using HPLC (high-performance liquid chromatography) with Xselect HSS T3 column (2.1 X 100 mm, 2.5㎛, Waters, USA) and ultrapure waterand acetonitrile as mobile phase A and B. Detection column temperature, injection volume and the flow rate were 35℃, 2 μL and 0.3 mL / minute respectively. The cordycepincontent of Cordyceps militarisproduced from medium mixed with vegetable and animal ingredientshigher than single ingredient. Moreover, through a variety of analyzes by varying the type and content of the medium additives, the cordycepinin Cordyceps militarisproduced from medium mixed with animal ingredientshighest. Furthermore, the cordycepincontent of a fruit body was higher than those of the a mycelium. Conclusion : These results provide a method for producing an high cordycepincontent of Cordyceps militarisas functional food ingredient.