Chemokine and chemokine receptor expression in paired peripheral blood mononuclear cells and synovial tissue of patients with rheumatoid arthritis, osteoarthritis, and reactive arthritis
2006
Background:
Chemokine receptorsand
chemokineshave a crucial role in leucocyte recruitment into inflamed tissue. Objective: To examine the expression of an extensive number of
chemokinesand receptors in a unique bank of paired samples of synovial tissue (ST) and peripheral blood (PB) from patients with different forms of arthritis to assist in identifying suitable targets for therapeutic intervention. Methods: Synovial biopsy specimens were obtained from 23 patients with rheumatoid arthritis (RA), 16 with osteoarthritis, and 8 with
reactive arthritis. ST
chemokine(
CCL2/MCP-1,
CCL5/RANTES,
CCL7/MCP-3,
CCL8/MCP-2,
CCL14/HCC-1,
CCL15/HCC-2,
CCL16/HCC-4),
chemokine receptor(
CCR1, CCR2b, CCR5,
CXCR4), and CD13 expression was analysed by immunohistochemistry and two colour immunofluorescence.
Chemokine receptorexpression (
CCR1,
CCR3, CCR5, CCR6, CCR7) on PB cells was studied by flow cytometry. Non-parametric tests were used for statistical analysis. Results: Abundant expression of
CCR1,
CXCR4, and CCR5 was found in all forms of arthritis, with a specific increase of
CCL5and
CCL15in RA.
CCL7,
CCL8,
CCL14,
CCL15, and
CCL16were detected for the first time in ST. The results for PB analysis were comparable among different arthritides. Interestingly, compared with healthy controls, significantly lower expression of
CCR1(p Discussion: A variety of
chemokinesand receptors might have an important role in several inflammatory
joint disorders. Although other receptors are involved as well, migration of
CCR1+ and CCR5+ cells towards the synovial compartment may play a part in the effector phase of various forms of arthritis.
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