Field cyanobacterial blooms producing retinoid compounds cause teratogenicity in zebrafish embryos
2019
Abstract Cyanobacteria routinely release potentially harmful bioactive compounds into the aquatic environment. Several recent studies suggested a potential link between the teratogenicity of effects caused by cyanobacteria and production of
retinoids. To investigate this relationship, we analysed the teratogenicity of field-collected cyanobacterial
bloomsamples by means of an in vivo zebrafish embryo test, an in vitro reporter gene bioassay and by the chemical analysis of
retinoids. Extracts of biomass from cyanobacterial
bloomswith the dominance of
Microcystis aeruginosaand Aphanizomenon klebahnii were collected from water bodies in the Czech Republic and showed significant
retinoid-like activity in vitro, as well as high degrees of teratogenicity in vivo. Chemical analysis was then used to identify a set of
retinoidsin ng per gram of dry weight concentration range. Subsequent fractionation and bioassay-based characterization identified two fractions with significant in vitro
retinoid-like activity. Moreover, in most of the
retinoidseluted from these fractions, teratogenicity with malformations typical for
retinoidsignalling disruption was observed in zebrafish embryos after exposure to the total extracts and these in vitro effective fractions. The zebrafish embryo test proved to be a sensitive toxicity indicator of the biomass extracts, as the teratogenic effects occurred at even lower concentrations than those expected from the activity detected in vitro. In fact, teratogenicity with
retinoid-like activity was detected at concentrations that are commonly found in biomasses and even in bulk water surrounding cyanobacterial
blooms. Overall, these results provide evidence of a link between
retinoid-like activity, teratogenicity and the
retinoidsproduced by cyanobacterial water
bloomsin the surrounding environment.
Keywords:
-
Correction
-
Source
-
Cite
-
Save
45
References
7
Citations
NaN
KQI