Reproducible Pharmaceutical Flash Purification Method Development by uPLC and HPLC

This research demonstrates that efficient and reproducible low pressure flash purifications can be enabled by scaling from generic analytical uPLC and HPLC conditions. Examples of challenging achiral reverse phase small molecule and cyclic peptides, and chiral separation examples demonstrate the success of this approach on a routine basis. Flash chromatography is a preferred approach for many chemists in pharmaceutical industry, although reverse phase mode is not often adopted. Improvements in manufacturing technology of flash column chemistries has enabled an efficient “scout to flash” open access workflow to enable chemists to choose this path in place of a HPLC semi-prep solution.