High-throughput single-cell whole-genome amplification through centrifugal emulsification and eMDA
2019
Single-cell
whole-genome sequencing(scWGS) is mainly used to probe intercellular genomic variations, focusing on the
copy number variationsor alterations and the single-nucleotide variations (SNVs) occurring within single cells. Single-cell whole-genome amplification (scWGA) needs to be applied before scWGS but is challenging due to the
low copy numberof DNA. Besides, many genomic variations are rare within a population of cells, so the throughput of currently available scWGA methods is far from satisfactory. Here, we integrate a one-step micro-capillary array (
MiCA)-based centrifugal droplet generation technique with emulsion
multiple displacement amplification(eMDA) and demonstrate a high-throughput scWGA method,
MiCA-eMDA.
MiCA-eMDA increases the single-run throughput of scWGA to a few dozen, and enables the assessment of
copy number variationsand alterations at 50-kb resolution. Downstream target enrichment further enables the detection of SNVs with 20% allele drop-out.
Keywords:
-
Correction
-
Source
-
Cite
-
Save
38
References
19
Citations
NaN
KQI