Mutational and phenotypic spectrum of OTOF -related auditory neuropathy in Koreans: eliciting reciprocal interaction between bench and clinics
2018
While
auditory neuropathy spectrum disorder(ANSD) is a
heterogeneous disorderand its management quite varies depending upon the etiology, even including self-resolution,
OTOFis an important molecular etiology of prelingual ANSD and has emerged as an attractive target for implementation of
precision medicinein terms of timing and prognosis prediction of auditory rehabilitation. However, to date, the literature is lacking in the genotype–phenotype relationship of this gene as well as efficient molecular
testing strategyin the clinic in many populations and to make things more complicated in Koreans, the most prevalent variant p.Arg1939Gln among Korean ANSD children frequently evaded detection by next generation sequencing (NGS), resulting in delayed genetic diagnosis and late cochlear implantation (CI). The aims of this study are to document the mutational and phenotypic spectrum of
OTOF-related ANSD (DFNB9) in the Korean population, further establishing genotype–phenotype correlation and proposing a set of the most commonly found
OTOFvariants to be screened first. Genetic diagnosis through the NGS-based sequencing was made on patients with ANSD in two tertiary hospitals. Genotype and phenotypes of eleven DFNB9 patients were reviewed. For data analysis, Mann–Whitney test and Fisher’s exact test were applied. This study disclosed four prevalent variants in Koreans: p.Arg1939Gln with an allele frequency of 40.9%, p.Glu841Lys (13.6%), p.Leu1011Pro and p.Arg1856Trp (9.1%). Three novel variants (c.4227 + 5G > C, p.Gly1845Glu, and p.Pro1931Thr) were identified. Interestingly, a significant association of p.Arg1939Gln with worse ASSR thresholds was observed despite consistently no ABR response. Ten of 11 DFNB9 patients received CI for auditory rehabilitation, showing favorable outcomes with more rapid improvement on early-CI group (age at CI ≤ 18 mo.) than late-CI group. This study included the largest Korean DFNB9 cohort to date and proposed a set of the most frequent four
OTOFvariants, allowing the potential prioritization of exons during
Sanger sequencing. Further, a significant association of p.Arg1939Gln homozygotes with poor residual hearing was observed. We may have to suspect p.Arg1939Gln homozygosity in cases of poor auditory thresholds in ANSD children with putative negative
OTOFvariants solely screened by NGS. Reciprocal feedback between bench and clinics regarding DFNB9 would complement each other.
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