A large-scale genome-wide enrichment analysis identifies new trait-associated genes, pathways and tissues across 31 human phenotypes*

Genome-wide association studies (GWAS) aim to identify genetic factors that are associated with complex traits. Standard analyses test individual genetic variants, one at a time, for association with a trait. However, variant-level associations are hard to identify (because of small effects) and can be difficult to interpret biologically. “Enrichment analyses” help address both these problems by focusing on sets of biologically-related variants . Here we introduce a new model-based enrichment analysis method that requires only GWAS summary statistics, and has several advantages over existing methods. Applying this method to interrogate 3,913 biological pathwaysand 113 tissue-based gene sets in 31 human phenotypes identifies many previously-unreported enrichments. These include enrichments of the endochondral ossificationpathway for adult height, the NFAT-dependent transcription pathway for rheumatoid arthritis, brain-related genes for coronary artery disease, and liver-related genes for late-onset Alzheimer9s disease. A key feature of our method is that inferred enrichments automatically help identify new trait-associated genes. For example, accounting for enrichment in lipid transport genes yields strong evidence for association between MTTP and low-density lipoprotein levels, whereas conventional analyses of the same data found no significant variants near this gene.