DEVELOPMENT AND VALIDATION OF HPTLC METHOD FOR ESTIMATION OF LACIDIPINE IN BULK AND PHARMACEUTICAL DOSAGE FORMS Research Article

A simple, specific, accurate and precise high performance thin layer chromatography (HPTLC) method has been developed for determination of Lacidipine in bulk and pharmaceutical dosage forms. The method uses aluminium plates coated with silica gel 60 F254 as stationary phase and Toulene : Ethylacetate (7.5 : 2.5 v / v) as mobile phase. Densitometric evaluation of the separated bands was performed at 239 nm using Camag TLC Scanner-3 with win CAT 1.4.4 software. The RF value of Lacidipine was 0.42 ± 0.02. The validated calibration range was 1000-3500 ng per spot (r 2 = 0.9991). The limit of detection was 0.011 ng / spot and Limit of quantitation was 0.034 ng / spot. Results of analysis were validated statistically and by recovery studies. The method was validated according to the ICH guidelines with respect to linearity, accuracy, precision and robustness. Thus the proposed method can be used successfully for routine analysis of Lacidipine from tablet formulations.