Activation of AZIN1 RNA editing is a novel mechanism that promotes invasive potential of cancer-associated fibroblasts in colorectal cancer
2019
Abstract Adenosine-to-
inosine(A-to-I)
RNA editingis a recently described epigenetic modification, which is believed to constitute a key oncogenic mechanism in human cancers. However, its functional role in cancer-associated
fibroblasts(CAFs) within the tumor microenvironment (TME) and its clinical significance remains unclear. Herein, we systematically analyzed a large cohort of 627 colorectal cancer (CRC) specimens, and investigated the expression pattern of ADAR1 and its biological significance on the antizyme inhibitor 1 ( AZIN1 )
RNA editinglevels. Both ADAR1 expression and AZIN1
RNA editinglevels were significantly elevated in CRC tissues vs. normal mucosa, and these findings correlated with the increased expression of mesenchymal markers, Vimentin (ρ = 0.44) and
Fibroblastactivation protein (ρ = 0.38). Intriguingly, ADAR1 expression was specifically upregulated in both cancer cells and
fibroblastsfrom cancerous lesions. Conditioned medium from cancer cells led to induction of ADAR1 expression and activation of AZIN1
RNA editingin
fibroblasts(p AZIN1 enhanced the invasive potential of
fibroblasts. In conclusion, we provide novel evidence that hyper-editing of AZIN1 enhances the invasive potential of CAFs within the TME in colon and is an important predictor of tumor invasiveness in CRC.
Keywords:
-
Correction
-
Source
-
Cite
-
Save
-
Machine Reading By IdeaReader
31
References
13
Citations
NaN
KQI