Evaluation of functional diversity of soil microbialcommunities - a case study
2018
In our case study, we measured the functional diversity of the
microbial communities of twelve soils from the small natural
area to assess if this assay is suitable for routine monitoring
of soil biological quality. We found the BIOLOG assay meets
especially practical benefits in routine monitoring of soils
being simple and quick assay. However, we confirmed the
ambiguity about the most appropriate analysis of the BIOLOG
multivariate data and about the best parameter, which can be
derived from the assay. The different analyses of the data were
examined and various parameters derived from the BIOLOG assay
were comparatively used to contribute to the discussion of how
the data should be evaluated. We showed that not-normalized raw
absorbances or trapezoid areas should be used for calculation
of diversity index if the inoculum was standardized. There was
no single answer to what parameter provided more correct
results in the multivariate statistical analysis. Evaluating at
least one not-normalized (e.g. trapezoid area) and one
normalized parameter (e.g. absorbances read in fixed AWCD) was
strongly suggested keeping in mind that they reveal different
information.
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