Comparative “phenol-omics” and gene expression analyses in peach (Prunus persica) skin in response to different postharvest UV-B treatments
2019
Abstract Ultraviolet-B (UV B) radiation impacts the plant behaviour in many ways, including modifying their
secondary metabolism. Although several studies have quantified the UV-B effects on
phenoliccomposition, most of them focused on leaves or investigated a limited amount of
phenolics. The present work aimed to investigate the
phenolicchanges after two postharvest UV-B treatments, 10 and 60 min (1.39 kJ m −2 and 8.33 kJ m −2 , respectively), on peach (
Prunuspersica cv Fairtime) fruit with a non-targeted, whole profiling approach, and targeted gene expression analysis on skin. After both UV-B exposures, peach fruit were harvested at 24 and 36 h for “
phenol-
omics” analysis, while additional 6 h and 12 h recovery times were used for gene expression analysis. Our results revealed that both UV-B exposures resulted in a decrease of several
phenoliccompounds, such as anthocyanins, after 24 h from the exposure. In contrast, the expression of the UV-B signalling components, the
phenylpropanoidbiosynthesis genes and their transcriptional regulators increased 6 h after the treatment, mostly with a UV-B-dose dependent behaviour, preceding an accumulation of most
phenolicsin both the UV-B treatments at 36 h compared to 24 h. Orthogonal projections to latent structures discriminant analysis (
OPLS-DA) revealed that flavonoids, particularly anthocyanins, were the main
phenolicsubclasses accumulated after UV-B exposure.
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