A pseudoknot is required for efficient translational initiation and regulation of the Escherichia coli rpsO gene coding for ribosomal protein S15.

Escherichia coli ribosomal proteinS15 down regulates its own synthesis by binding to its mRNA in a region overlapping the ribosome binding site, called the translational operator. This binding stabilizes a pseudoknotstructure that exists in equilibrium with two stem–loopstructures. When synthesized in excess over 16S rRNA, S15 binds to its translational operatorand traps the ribosome on its loading site in a transient state, preventing the formation of the active ternary (30S–mRNA–rRNAfMet) complex. This inhibition can be suppressed by 16S rRNA, which displaces S15 from the mRNA. An extensive mutational analysis showed that the pseudoknotis the structural element required for S15 recognition and in vivo translational control. Specific sequence determinantsare located in limited regions of the structure formed by the pseudoknot. An unexpected result is that the pseudoknotcan exist in a variety of topologically equivalent structures recognizable and shapable by S15. Based on footprinting experiments ...