Assays for laboratory confirmation of novel human coronavirus (hCoV-EMC) infections
2012
We present a rigorously validated and highly sensitive confirmatory real-time RT-PCR
assay(1A
assay) that can be used in combination with the previously reported upE
assay. Two additional RT-PCR
assaysfor sequencing are described, targeting the RdRp gene (RdRpSeq
assay) and N gene (NSeq
assay), where an insertion/deletion polymorphism might exist among different hCoV-EMC strains. Finally, a simplified and biologically safe protocol for detection of antibody response by immunofluorescence microscopy was developed using
convalescentpatient serum.
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