How haptophytes microalgae mitigate vitamin B 12 limitation

Vitamin B12( cobalamin) can control phytoplankton development and community composition, with around half of microalgal species requiring this vitamin for growth. B12 dependency is determined by the absence of cobalamin-independent methionine synthaseand is unrelated across lineages. Despite their important role in carbon and sulphur biogeochemistry, little is known about haptophytesutilization of vitamin B12and their ability to cope with its limitation. Here we report the first evaluation of B12 auxotrophyamong this lineage based on molecular data of 19 species from 9 families. We assume that all species encode only a B12-dependent methionine synthase, suggesting ubiquitous B12 auxotrophyin this phylum. We further address the effect of different B12 limitations on the molecular physiology of the model haptophyteTisochrysis lutea. By coupling growth assays in batch and chemostatto cobalaminquantification and expression analyses, we propose that haptophytesuse three strategies to cope with B12 limitation. Haptophytesmay assimilate dissolved methionine, finely regulate genes involved in methioninecycle and B12 transport and/or limit B12 transport to the mitochondrion. Taken together, these results provide better understanding of B12 metabolism in haptophytesand represent valuable data for deciphering how B12-producing bacteria shape the structure and dynamics of this important phytoplankton community.