Inhibition of DLX4 Promotes Apoptosis in Choriocarcinoma Cell Lines
2006
Homeodomain (HDM) proteins encoded by
homeobox(
HBX) genes represent a large family of transcriptional factors that control differentiation and development in certain cell types. DLX4 is a member of Distal-less (DLX) family of
HBXgenes. Recent studies have demonstrated that abnormal expression of DLX4 is present in several types of human tumors, such as breast cancer, leukemia and colon cancer. In the present study, we investigated DLX4 mRNA and protein expression in both normal placental tissues and human
choriocarcinomacell lines. Also, using RNA interference (RNAi) technique, we knocked down the expression of DLX4 and examined apoptosis in JEG-3 cells. Our studies demonstrated that DLX4 RNAi inhibited DLX4 mRNA expression and decreased DLX4 protein mass specifically and
effectively,
potentiallyenhancing apoptosis. Moreover, we examined expression of caspase-3 and
caspase-8, and found that both caspases were increased after DLX4 knockdown. However, DLX4 RNAi did not influence Bax expression in JEG-3 cells. In conclusion, this study suggests that DLX4 may be involved in the survival of human
choriocarcinomacells, which may be mediated by the inhibition of apoptosis. The detailed mechanism needs further investigation.
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