Complete genome sequence of Agrobacterium pusense VsBac-Y9, a bacterial symbiont of the dark septate endophytic fungus Veronaeopsis simplex Y34 with potential for improving fungal colonization in roots
2018
Abstract A
Rhizobium-related bacterium (
Rhizobiumsp. VsBac-Y9) is a symbiont living with the
dark septate endophytic(DSE)
fungusVeronaeopsis
simplexY34. Co-inoculation of
Rhizobiumsp. VsBac-Y9 with V.
simplexY34 improves the fungal colonization of tomato roots, resulting in a significant increase in aboveground biomass. This study sequenced the complete genome of this V.
simplex-helper bacterium using the PacBio and Illumina MiSeq platforms. Hybrid assembly using SPAdes outputted a circular chromosome, a linear chromid, and a circular plasmid for a total genome 5,321,211 bp in size with a G + C content of 59.2%. Analysis of concatenated
housekeeping genes( atpD-dnaK-
groEL-
lepA-recA-
rpoB-thrE ) and calculation of average nucleotide identity, showed that VsBac-Y9 was affiliated with the species Agrobacterium pusense (syn.
Rhizobiumpusense ). Genome analysis revealed that A. pusense VsBac-Y9 contains a series of genes responsible for the host interactions with both
fungusand plant. Such genomic information will provide new insights into developing co-inoculants of
endophytic
fungusand its symbiotic bacterium in future agricultural innovation.
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