IDDF2018-ABS-0124 Identification of MIR-148A in plasma as a potential noninvasive biomarker for hepatocellular carcinoma

Background Detection of microRNA (miRNA) aberrations in the peripheral plasma is a new approach for hepatocellular carcinoma (HCC) screening. The aim of this study was to characterise miR-148a in the peripheral plasma as a non-invasive biomarker for diagnosis of HCC. Methods Plasma-based miR148a analysis was performed on 346 plasma samples, including 155 HCCs, 96 liver cirrhosis and 95 healthy controls by using quantitative Real-Time PCR (qRT-PCR). Subsequently, plasma-based miR148a levels were validated in 97 pairs of HCC followed-up after removal of the primary tumour. Finally, Receiver operating characteristics (ROC) curves were generated to confirm predicting the value of plasma-based miR148a in HCC. Results Plasma-based miR-148a expression was significantly lower in 155HCCs compared to 96 liver cirrhosis (p Conclusions Plasma-based miR-148a can be applied as a potential non-invasive biomarker for HCC screening, especially for HCC with negative or less expressing AFP, which can make up AFP deficiency in predicting HCC occurrence. Notes This project was funded by Application Project of Clinical Features in Capital City (Z141107002514507).