Type-1 Polarized Dendritic Cells Loaded with Apoptotic Prostate Cancer Cells Are Potent Inducers of CD8 + T cells against Prostate Cancer Cells and Defined Prostate Cancer-Specific Epitopes

Background—In order to develop improved vaccines for patients with recurrent prostate cancer (PCa), we tested the feasibility of using type-1-polarized dendritic cells (DC1s) to cross-present antigens from allogeneic PCa cells and to induce functional CD8 + T cell responses against PCa cells and against defined MHC class I-restricted PCa-relevant epitopes. Methods—Monocyte-derived DCs from PCa patients were matured using the “standard” cytokine cocktail (IL-1β/TNFα/IL-6/PGE2) or using the αDC1-polarizing cocktail (IL-1β/TNFα/ IFNα/IFNγ/poly-I:C), loaded with UV-irradiated LNCaP cells, and used to sensitize autologous CD8 + T cells.