DHA enrichment of the red earthworm Eisenia fetida for improving its potential as dietary source for aquaculture

Abstract This study aimed at assessing the potential of omega-3 fatty acids enrichment in the red earthworm Eisenia fetida. Specifically, the investigation focused on the effects of a commercial enrichment emulsion (ES) supplied at graded doses (0, 10, 25 and 50 g ES/100 g of compost) and at different enrichment times (0, 12, 24, 48 and 96 h) on the resulting fatty acids composition of red earthwormreared with or without additional bedding materials. In the first experiment (bed-free technique), inclusion of ES, in the highest tested dose (50 g/100 g compost), exerted stress on the earthwormsresulting in high mortality, while the use of bedding in the second experiment led to safer but slightly reduced enrichment. Dominant fatty acids in earthwormswere found to be PUFA (51.08–53.04 mol%), followed by MUFA (25.95–26.90 mol%) and SFA (21.16–22 mol%). Non-enriched earthwormscontained some of the PUFA, important for cultured marine species, including 18:2n-6, 18:3n-3 and 20:5n-3 (EPA), whereas in contrast, 22:6n-3 (DHA) is very low. Overall the results of this study have, for the first time, showed that LC-PUFA, and especially DHA, contents of E. fetida can be enriched to levels much higher than those of the control (non-enriched) groups. In bed-free technique, DHA accumulation in the worms increased from 0.31 mol% (0 h) to 14.38–14.63 mol% at 12 h, 9.65–9.81 mol% at 24 h, 10.65–15.41 mol% at 48 h and finally 15.81–18.31 mol% at 96 h. In the bedding technique, DHA also rose from the base levelto 5.27–5.97 mol% at 12 h, 5.25–8.60 mol% at 24 h, 11.06–13.01 mol% at 48 h and finally 14.05–17.14 mol% at 96 h. In the bedding technique, DHA increased linearly for up to 9.6 fold, while this increase was fluctuating in the bed-free technique, reaching 8.7 fold over the non-enriched controls. Throughout the experiments, n-3/n-6 ratios rose from 1.35–1.56 in the controls to 3.38–3.72 in the enriched-groups. As a result, 12-h enrichment time is suggested to be suitable for a fast boosting of DHA in the earthwormswith bed-free technique, while a longer period of up to 96 h is recommended with bedding technique at optimal dose of 25 g ES inclusion into 100 g compost. The present study showed that earthworm( E.fetida ) can be successfully enriched in its DHA content; making it a novel and easily producible feed resource, particularly as broodstockfeed, in aquatic hatcheries.