The Expression of Brain-Type Fatty Acid Binding Protein (FABP7) in Rainbow Trout (Oncorhynchus Mykiss)

The Fatty acid-binding proteins (FABP) is a 14-15kDa intracellular protein which belong to large multigene family of intracellular lipid-binding proteins and play a important role in lipid uptake, transport lipid homeostasis. In this study, the rainbow trout FABP7 (GenBank accession No. JN413683) and actin (GenBank accession No. AF330142) were used to design primers for analyzing the expression during temperature challenge using qRT-PCR approach. The FABP7 are detected in liver, intestine and spleen in triploid, golden and diploid rainbow trout. After the temperature dropped from 10°C to 3°C, the relatively expression peaks at 6hr(triploid), 12hr (golden) in liver and 2hr (diploid) in intestine, the FABP7 reduced from 2hr and 6hr to 24hr in diploid and triploid rainbow trout in intestine, the FABP7 increased from 0hr to 24hr in diploid rainbow trout. After the temperature raise from 10°C to 18°C, the relatively expression peaks at 24hr (triploid) in intestine and 24hr (golden) in spleen, the FABP7 reduced from 2hr to 12hr (diploid and triploid) in intestine and 2hr to 12hr (diploid) and from and 0hr to 6hr (triploid) in spleen, the FABP7 increased from 2hr to 12hr (golden) in intestine. Overall, these results demonstrate the expression of FABP was received impact by changes of temperature.