Clinical and biological predictors of response to standardised paediatric colitis therapy (PROTECT): a multicentre inception cohort study
2019
Summary Background Lack of evidence-based outcomes data leads to uncertainty in developing treatment regimens in children who are newly diagnosed with
ulcerative colitis. We hypothesised that pretreatment clinical, transcriptomic, and microbial factors predict disease course. Methods In this inception cohort study, we recruited paediatric patients aged 4–17 years with newly diagnosed
ulcerative colitisfrom 29 centres in the USA and Canada. Patients initially received standardised
mesalazineor corticosteroids, with pre-established criteria for escalation to immunomodulators (ie, thiopurines) or anti-tumor necrosis factor-α (TNFα) therapy. We used RNA sequencing to define rectal gene expression before treatment, and 16S sequencing to characterise rectal and faecal microbiota. The primary outcome was week 52 corticosteroid-free remission with no therapy beyond
mesalazine. We assessed factors associated with the primary outcome using logistic regression models of the per-protocol population. This study is registered with ClinicalTrials.gov, number NCT01536535. Findings Between July 10, 2012, and April 21, 2015, of 467
patients recruited, 428 started medical therapy, of whom 400 (93%) were evaluable at 52 weeks and 386 (90%) completed the study period with no protocol violations. 150 (38%) of 400 participants achieved week 52 corticosteroid-free remission, of whom 147 (98%) were taking
mesalazineand three (2%) were taking no medication. 74 (19%) of 400 were escalated to immunomodulators alone, 123 (31%) anti-TNFα therapy, and 25 (6%)
colectomy. Low baseline clinical severity, high baseline haemoglobin, and week 4 clinical remission were associated with achieving week 52 corticosteroid-free remission (n=386, logistic model area under the curve [AUC] 0·70, 95% CI 0·65–0·75; specificity 77%, 95% CI 71–82). Baseline severity and remission by week 4 were validated in an independent cohort of 274 paediatric patients with newly diagnosed
ulcerative colitis. After adjusting for clinical predictors, an antimicrobial peptide
gene signature(odds ratio [OR] 0·57, 95% CI 0·39–0·81; p=0·002) and abundance of
Ruminococcaceae(OR 1·43, 1·02–2·00; p=0·04), and
Sutterella(OR 0·81, 0·65–1·00; p=0·05) were independently associated with week 52 corticosteroid-free remission. Interpretation Our findings support the utility of initial clinical activity and treatment response by 4 weeks to predict week 52 corticosteroid-free remission with
mesalazinealone in children who are newly diagnosed with
ulcerative colitis. The development of personalised clinical and biological signatures holds the promise of informing
ulcerative colitistherapeutic decisions. Funding US National Institutes of Health.
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