Studies on microRNA in Pediatric Tuberculosis

Background: Pediatric tuberculosis (TB) is still a major problem in the world leads to adverse complications. Research of microRNA(miRNA) conducted to study and identify specific miRNA expression among active TB children specifically in Indonesia. This suggests that miRNA can be one of the diagnostic tools and therapy for pediatric TB that needs to be developed. Aims: To study spesific miRNA expression in pediatric active TB in Indonesia using microarray profiling. Methods: This research was an observational analytic study using a cross sectional study design. The study used whole blood of healthy control children who had never received BCG immunization and children diagnosed with active TB. Identified miRNAs expression profile with microarray analysis. Results: Four Active TB and 2 healthy control children were included in this study. Extraction of RNA with whole blood were done and profiled with microarray methods. Fold change >2 or