Proteogenomic insights into the intestinal parasite Blastocystis sp. subtype 4 isolate WR1

Blastocystissp. is known for years as a highly prevalent anaerobic eukaryotic parasite of humans and animals. Several monophyletic clades have been delineated based on molecular data and the occurrence of each subtype (ST) in humans and/or animal hosts has been documented. The genome of several representatives has been sequenced revealing specific traits such as an intriguing 3’-end processing of primary transcripts. Here, a first high-throughput proteomics dataset acquired on this difficult to cultivate parasite is presented for the zoonotic ST4 isolate WR1. Amongst the 2,766 detected proteins, we highlighted the role of a small ADP ribosylation factor(Arf) GTP-binding protein involved in intracellular traffic as major regulator of vesicle biogenesis and a voltage-dependent anion-selective channel protein because both were unexpectedly highly abundant. We show how these data may be used for gaining proteogenomicsinsights into Blastocystissp. specific molecular mechanisms. We evidenced for the first time by proteogenomicsa functional termination codon derived from transcript polyadenylationfor seven different key cellular components. This article is protected by copyright. All rights reserved